Rebaudioside A, one of the major compounds biosynthesized in the plant is the main natural sweetener commercialized from this source. 1333/2008 of the European Parliament and of the Council with Regard to Steviol Glycosides Off. 1131/2011: Amending Annex II to Regulation (EC) No. , 6 6 The European Commission Commission Regulation (EU) No. GRN 000304, CFSAN/Office of Food Additive Safety GRAS Associates, LLC: Bend, 2010. Food and Drug Administration Agency Response Letter GRAS Notice No. GRN 000252, CFSAN/Office of Food Additive Safety Whole Earth Sweetener Company LLC: Chicago, IL, 2008 U. rebaudiana were accepted in the United States of America (USA) in 2008 and in the European Market in 2011. In Stevia, The Genus Stevia, Medicinal and Aromatic Plants-Industrial Profiles, Vol.

However, crude plant extracts were not employed as sweetening agents until 1971 in Japan and stevioside was accepted officially in Korea as an alternative sweetener in 1976.

Sur le Principe SucrГ© des Feuilles de KaГў-HГЄ-Г‰ (Stevia Rebaundiana B) Academie des Sciences Paris Comptes Rendus (Parts 192): Paris, 1931, pp. The main aglycone of these glycosides is steviol and the first steviol glycosides, stevioside and rebaudioside A, were described from S. These compounds are several hundred times more sweet than sucrose.

1-17.Дiterpene glycosides with an ent-kaurene skeleton are recognized as the metabolites from S. The plant grows up to 1 m high and is native to Brazil and Paraguay but its cultivars are now grown commercially all over the world. Stevia rebaudiana (Bertoni) Bertoni (Asteraceae) has been used as a sweetener for centuries by Guarani indigenous due to the occurrence of diterpene glycosides in its leaves. Stevia rebaudiana diterpene glycoside HPLC technique scale-up enhanced normal phase preparative chromatography The development of these analytical and preparative liquid chromatography techniques allowed the isolation of several steviol glycosides in gram quantities at relatively low cost. Additionally, we also developed an easy and useful way for predicting yields and composition of the pools resulting from the preparative chromatography. Strategies for scale up of the purification process of steviol glycosides to make it more economic are also summarized, emphasizing the advantages of using high efficiency normal phase chromatography in relation with reverse phase. The advantages and disadvantages of each method for resolving the very polar and less polar elution regions are demonstrated. Different stationary phases/columns were used: reverse phase C-18, amino, HILIC (hydrophilic interaction chromatography), Sepaxdiol and Synergi. Herein, we describe the development of several routine HPLC (high performance liquid chromatography) analytical techniques to better separate steviol glycosides. Stevia rebaudiana is a plant well-known as a source of dozens of high potency non-caloric natural sweeteners, many of them still uncharacterized.

Solvent cut off wavelength